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ba f3 gp130 cells  (DSMZ)


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    DSMZ ba f3 gp130 cells
    Ba F3 Gp130 Cells, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ba+f3+gp130+cells/BA%2FF3/pm40995978-206-6-25
    Average 96 stars, based on 371 article reviews
    ba f3 gp130 cells - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Transduction:

    Article Title: Identification of Canonical Tyrosine-dependent and Non-canonical Tyrosine-independent STAT3 Activation Sites in the Intracellular Domain of the Interleukin 23 Receptor
    Article Snippet: .. Cells and Reagents—Ba/F3-gp130 cells transduced with human gp130 were kindly provided by Immunex (Seattle, WA) (32), HeLa cells (ACC-57) were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany), and the packaging cell line Phoenix-Eco was from U. Klingmüller (DKFZ, Heidelberg, Germany) (33). .. All cell lineswere grown inDMEMhigh glucose culture medium (Invitrogen) supplemented with 10% fetal calf serum (Invitrogen), 60 mg/liter penicillin, and 100 mg/liter streptomycin (Genaxxon Bioscience GmbH, Ulm, Germany) at 37 °C with 5% CO2 in a water-saturated atmosphere.

    Enzyme-linked Immunosorbent Assay:

    Article Title: iBody-mediated tuning of synthetic cytokine receptor activation via rational nanobody interface engineering.
    Article Snippet: Ba/F3, HEK293T, and PhoenixEco cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) high glucose (GIBCO, Life Technologies, Darmstadt, Germany), supplemented with 10% fetal calf serum (FCS) (GIBCO, Life Technologies, Darmstadt, Germany) and 1% penicillin-streptomycin (60 mg/l penicillin and 100 mg/l streptomycin) (Genaxxon Bioscience GmbH, Ulm, Germany). .. The growth medium for Ba/F3 or Ba/ F3-gp130 cells was further supplemented with 0.2% conditioned medium from IL-3-secreting WEHI3B murine myelomonocytic leukemic cells (ACC 26, DSMZ) called WEHI, or from a stable CHO-K1 cell clone secreting Hyper-IL-6 (stock solution approximately 10 μg/ml, as determined by ELISA) called Hy. ..

    Article Title: iBody-mediated tuning of synthetic cytokine receptor activation via rational nanobody interface engineering
    Article Snippet: Ba/F3, HEK293T, and Phoenix-Eco cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) high glucose (GIBCO, Life Technologies, Darmstadt, Germany), supplemented with 10% fetal calf serum (FCS) (GIBCO, Life Technologies, Darmstadt, Germany) and 1% penicillin-streptomycin (60 mg/l penicillin and 100 mg/l streptomycin) (Genaxxon Bioscience GmbH, Ulm, Germany). .. The growth medium for Ba/F3 or Ba/F3-gp130 cells was further supplemented with 0.2% conditioned medium from IL-3-secreting WEHI-3B murine myelomonocytic leukemic cells (ACC 26, DSMZ) called WEHI, or from a stable CHO-K1 cell clone secreting Hyper-IL-6 (stock solution approximately 10 μg/ml, as determined by ELISA) called Hy. ..

    Article Title: Targeting the major pro-inflammatory interleukin-6-type cytokine receptor gp130 by antagonistic single domain antibodies
    Article Snippet: Ba/F3, HEK293T and Phoenix Eco cells were cultured in Dulbecco’s modified Eagle’s Medium (DMEM) high glucose culture medium (GIBCO, Life Technologies, Darmstadt, Germany), containing 10% fetal calf serum (FCS) (GIBCO, Life Technologies, Darmstadt, Germany), 60 mg/l penicillin (P) and 100 mg/l streptomycin (S) (1% P/S) (Genaxxon Bioscience GmbH, Ulm, Germany). .. Growth medium of Ba/F3 or Ba/F3-gp130 cells additionally include 0.2% of conditioned medium from IL-3 secreting WEHI-3B murine myelomonocytic leukemic cells (ACC 26, DSMZ), or a stable clone of CHO-K1 cells secreting Hyper-IL-6 respectively (stock solution approximately 10 μg/ml as determined by ELISA). ..

    Stable Transfection:

    Article Title: Targeting the major pro-inflammatory interleukin-6-type cytokine receptor gp130 by antagonistic single domain antibodies
    Article Snippet: Ba/F3, HEK293T and Phoenix Eco cells were cultured in Dulbecco’s modified Eagle’s Medium (DMEM) high glucose culture medium (GIBCO, Life Technologies, Darmstadt, Germany), containing 10% fetal calf serum (FCS) (GIBCO, Life Technologies, Darmstadt, Germany), 60 mg/l penicillin (P) and 100 mg/l streptomycin (S) (1% P/S) (Genaxxon Bioscience GmbH, Ulm, Germany). .. Growth medium of Ba/F3 or Ba/F3-gp130 cells additionally include 0.2% of conditioned medium from IL-3 secreting WEHI-3B murine myelomonocytic leukemic cells (ACC 26, DSMZ), or a stable clone of CHO-K1 cells secreting Hyper-IL-6 respectively (stock solution approximately 10 μg/ml as determined by ELISA). ..



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    c19s130Fc blocks IL-6 trans -signaling. (A) SPR analysis of HIL-6 binding to c19s130Fc. c19s130Fc was immobilized on a protein A chip, and increasing concentrations of HIL-6 were injected. Sensorgrams in response units (RU) over time are depicted as colored lines, and global fit data are displayed as black lines. K D , equilibrium dissociation constant; k d , dissociation constant. (B) <t>Ba/F3-gp130</t> cells were stimulated with 100 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of increasing c19s130Fc, cs130Fc, or VHH72Fc concentrations. At 72 h poststimulation, cellular proliferation was detected using CellTiter-Blue. Assay results are representative of data from three independent experiments. (C) Western blot analysis of Ba/F3-gp130 cells stimulated for 30 min with 8 nM IL-6 and 1 nM sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pERK, and ERK. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included. (D) Western blot analysis of Vero cells stimulated for 30 min with 400 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pSTAT1, and STAT1. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included.
    Ba F3 Gp130 Hace2 Gp130 Cells, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ba f3 gp130 cells  (DSMZ)
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    DSMZ ba f3 gp130 cells
    c19s130Fc blocks IL-6 trans -signaling. (A) SPR analysis of HIL-6 binding to c19s130Fc. c19s130Fc was immobilized on a protein A chip, and increasing concentrations of HIL-6 were injected. Sensorgrams in response units (RU) over time are depicted as colored lines, and global fit data are displayed as black lines. K D , equilibrium dissociation constant; k d , dissociation constant. (B) <t>Ba/F3-gp130</t> cells were stimulated with 100 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of increasing c19s130Fc, cs130Fc, or VHH72Fc concentrations. At 72 h poststimulation, cellular proliferation was detected using CellTiter-Blue. Assay results are representative of data from three independent experiments. (C) Western blot analysis of Ba/F3-gp130 cells stimulated for 30 min with 8 nM IL-6 and 1 nM sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pERK, and ERK. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included. (D) Western blot analysis of Vero cells stimulated for 30 min with 400 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pSTAT1, and STAT1. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included.
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    ba/f3-gp130 cells  (Immunex Corporation)
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    c19s130Fc blocks IL-6 trans -signaling. (A) SPR analysis of HIL-6 binding to c19s130Fc. c19s130Fc was immobilized on a protein A chip, and increasing concentrations of HIL-6 were injected. Sensorgrams in response units (RU) over time are depicted as colored lines, and global fit data are displayed as black lines. K D , equilibrium dissociation constant; k d , dissociation constant. (B) <t>Ba/F3-gp130</t> cells were stimulated with 100 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of increasing c19s130Fc, cs130Fc, or VHH72Fc concentrations. At 72 h poststimulation, cellular proliferation was detected using CellTiter-Blue. Assay results are representative of data from three independent experiments. (C) Western blot analysis of Ba/F3-gp130 cells stimulated for 30 min with 8 nM IL-6 and 1 nM sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pERK, and ERK. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included. (D) Western blot analysis of Vero cells stimulated for 30 min with 400 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pSTAT1, and STAT1. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included.
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    c19s130Fc blocks IL-6 trans -signaling. (A) SPR analysis of HIL-6 binding to c19s130Fc. c19s130Fc was immobilized on a protein A chip, and increasing concentrations of HIL-6 were injected. Sensorgrams in response units (RU) over time are depicted as colored lines, and global fit data are displayed as black lines. K D , equilibrium dissociation constant; k d , dissociation constant. (B) <t>Ba/F3-gp130</t> cells were stimulated with 100 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of increasing c19s130Fc, cs130Fc, or VHH72Fc concentrations. At 72 h poststimulation, cellular proliferation was detected using CellTiter-Blue. Assay results are representative of data from three independent experiments. (C) Western blot analysis of Ba/F3-gp130 cells stimulated for 30 min with 8 nM IL-6 and 1 nM sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pERK, and ERK. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included. (D) Western blot analysis of Vero cells stimulated for 30 min with 400 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pSTAT1, and STAT1. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included.
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    Image Search Results


    c19s130Fc blocks IL-6 trans -signaling. (A) SPR analysis of HIL-6 binding to c19s130Fc. c19s130Fc was immobilized on a protein A chip, and increasing concentrations of HIL-6 were injected. Sensorgrams in response units (RU) over time are depicted as colored lines, and global fit data are displayed as black lines. K D , equilibrium dissociation constant; k d , dissociation constant. (B) Ba/F3-gp130 cells were stimulated with 100 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of increasing c19s130Fc, cs130Fc, or VHH72Fc concentrations. At 72 h poststimulation, cellular proliferation was detected using CellTiter-Blue. Assay results are representative of data from three independent experiments. (C) Western blot analysis of Ba/F3-gp130 cells stimulated for 30 min with 8 nM IL-6 and 1 nM sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pERK, and ERK. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included. (D) Western blot analysis of Vero cells stimulated for 30 min with 400 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pSTAT1, and STAT1. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included.

    Journal: Journal of Virology

    Article Title: A Hybrid Soluble gp130/Spike-Nanobody Fusion Protein Simultaneously Blocks Interleukin-6 trans -Signaling and Cellular Infection with SARS-CoV-2

    doi: 10.1128/jvi.01622-21

    Figure Lengend Snippet: c19s130Fc blocks IL-6 trans -signaling. (A) SPR analysis of HIL-6 binding to c19s130Fc. c19s130Fc was immobilized on a protein A chip, and increasing concentrations of HIL-6 were injected. Sensorgrams in response units (RU) over time are depicted as colored lines, and global fit data are displayed as black lines. K D , equilibrium dissociation constant; k d , dissociation constant. (B) Ba/F3-gp130 cells were stimulated with 100 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of increasing c19s130Fc, cs130Fc, or VHH72Fc concentrations. At 72 h poststimulation, cellular proliferation was detected using CellTiter-Blue. Assay results are representative of data from three independent experiments. (C) Western blot analysis of Ba/F3-gp130 cells stimulated for 30 min with 8 nM IL-6 and 1 nM sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pERK, and ERK. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included. (D) Western blot analysis of Vero cells stimulated for 30 min with 400 ng/mL IL-6 and 200 ng/mL sIL-6R in the presence of the indicated concentrations of c19s130Fc and cs130Fc. Prior to stimulation, IL-6, sIL-6R, and inhibitors were incubated separately for 30 min. Western blots were stained for pSTAT3, STAT3, pSTAT1, and STAT1. Western blots are representative of results from three independent experiments. Controls for unstimulated cells (−), cells in the absence of c19s130Fc (+), and stimulation with HIL-6 are included.

    Article Snippet: Flow cytometry inhibition experiments were conducted with 5 × 10 5 Ba/F3-gp130-hACE2-gp130 cells in the presence of 5 nM S-RBD, inhibitory proteins, and the primary antibody (antispike, catalog number 40150-R007; Sino Biological) (1:50) for 1 h at room temperature.

    Techniques: Binding Assay, Injection, CtB Assay, Western Blot, Incubation, Staining

    c19s130Fc prevents S-RBD binding to overexpressed ACE2 in Ba/F3 cells. (A) Flow cytometric analysis of cell surface expression of hACE2-gp130 (red population) in Ba/F3-gp130 cells detected by hACE2 antibody. The blue population indicates Ba/F3-gp130 cells incubated without hACE antibody (control). (B) Flow cytometric analysis of S-RBD binding to Ba/F3-ACE2 cells. Following incubation with S-RBD, S-RBD binding was detected (blue area) using an anti-spike S1 antibody (Sino Biological). Ba/F3-gp130-hACE2-gp130 cells without treatment served as controls (red area). (C to E) S-RBD binding in the presence of increasing concentrations of c19s130Fc, VHH72Fc, or cs130Fc. A total of 20,000 events were recorded, and the cell count was normalized. Histograms are representative of results from 3 independent experiments.

    Journal: Journal of Virology

    Article Title: A Hybrid Soluble gp130/Spike-Nanobody Fusion Protein Simultaneously Blocks Interleukin-6 trans -Signaling and Cellular Infection with SARS-CoV-2

    doi: 10.1128/jvi.01622-21

    Figure Lengend Snippet: c19s130Fc prevents S-RBD binding to overexpressed ACE2 in Ba/F3 cells. (A) Flow cytometric analysis of cell surface expression of hACE2-gp130 (red population) in Ba/F3-gp130 cells detected by hACE2 antibody. The blue population indicates Ba/F3-gp130 cells incubated without hACE antibody (control). (B) Flow cytometric analysis of S-RBD binding to Ba/F3-ACE2 cells. Following incubation with S-RBD, S-RBD binding was detected (blue area) using an anti-spike S1 antibody (Sino Biological). Ba/F3-gp130-hACE2-gp130 cells without treatment served as controls (red area). (C to E) S-RBD binding in the presence of increasing concentrations of c19s130Fc, VHH72Fc, or cs130Fc. A total of 20,000 events were recorded, and the cell count was normalized. Histograms are representative of results from 3 independent experiments.

    Article Snippet: Flow cytometry inhibition experiments were conducted with 5 × 10 5 Ba/F3-gp130-hACE2-gp130 cells in the presence of 5 nM S-RBD, inhibitory proteins, and the primary antibody (antispike, catalog number 40150-R007; Sino Biological) (1:50) for 1 h at room temperature.

    Techniques: Binding Assay, Expressing, Incubation, Cell Counting